Anti-LPS mAbs

Cat. No.

MABL-2277

Name

Anti-LPS mAbs

Clone No.

AFD- CK3 (BP1 7F7)

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA

Species Reactivity

Burkholderia pseudomallei, Burkholderia thailandensis

Basic Information

Specificity

This antibody binds the surface   lipopolysaccharide of Burkholderia pseudomallei and Burkholderia   thailandensis. Burkholderia pseudomallei (BP), is Recently, BP has been   recognized as a major cause of community-acquired septicemia, resulting in   significant mortalitythe causative agent of melioidosis (Whitmore’s disease),   is a gram-negative, facultative anaerobic, motile bacillus commonly found in   the soil and stagnant waters. BP infection is often due to either direct   inoculation into wounds and skin abrasions or inhalation of contaminated   materials. Recently, BP has been recognized as a major cause of community-   acquired septicemia, resulting in significant mortality.

Alternative Name

Lipopolysaccharide; Burkholderia spp;   Burkholderia pseudomallei; BP; Burkholderia thailandensis, melioidosis;   Whitmore’s disease

UniProt

Immunogen

The original antibody was generated by   immunizing mice with various antigen preparations from B. pseudomallei (ATCC 23343).

Application Notes

This antibody can be   used for the detection of heat killed, sodium periodate treated, proteinase K   treated or 0.02% SDS treated Burkholderia pseudomallei and Burkholderia   thailandensis in a sample using ELISA. It was reported that this antibody   could recognize 34, 38∼52 kDa polypeptide antigens from   Burkholderia pseudomallei and 34, 38∼60 kDa polypeptide antigens from   Burkholderia thailandensis in a western blot assay (PMID: 21573027).

Antibody First   Published

Yong-Kim et al. Production and   characterization of chimeric monoclonal antibodies against Burkholderia   pseudomallei and B. mallei using the DHFR expression system. PLoS One. 2011   May 9;6(5):e19867. PMID:21573027

Note on publication

Describes the generation and   characterization of chimeric antibodies against Burkholderia pseudomallei and   B. mallei.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.