Key features and details | |
Cat. No. | MABL-2279 |
Name | Anti-LPS mAbs |
Clone No. | AFD- S20 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralize, ELISA |
Species Reactivity | 06ad P. aeruginosa |
Basic Information | |
Specificity | S20 binds to the polysaccharide (PS) portion of the lipopolysaccharide O side chain of a common pathogenic serogroup of P. aeruginosa, 06ad. The antibody also binds Fisher Devlin immunotype 1 P. aeruginosa, (considered part of the 06 serogroup) but there is no cross-reactivity with LPSs from other P. aeruginosa serotypes (strains IATS 011, IATS 016, 170003, and PAO1 Holloway). P. aeruginosa is a human pathogen, which poses a serious risk to individuals with weak immune systems. LPS is a serotypic marker present on the surface of the bacterium. |
Alternative Name | lipopolysaccharide; lipopolysaccharide O side chain |
UniProt | |
Immunogen | Ig-inactivated transgenic mice possessing human heavy and light Igs (strain XMG2) were immunized with heat-killed 06ad P. aeruginosa. The supernatants from the derived hybridomas were screened for IgG mAb production against the 06ad high molecular weight LPS O-side-chain using ELISA. |
Application Notes | S20 human MAb is highly opsonic (complement-dependent) for the uptake and killing of live and heat-killed P. aeruginosa by fresh human PMN ( polymorphonuclear leukocytes). BALB/c mice rendered neutropenic challenged with 06ad P. aeruginosa saw 100% protection from mortality. S20 can also be used to detect 06ad LPS by ELISA. |
Antibody First Published | Hemachandra Human Monoclonal Antibodies against Pseudomonas aeruginosa Lipopolysaccharide Derived from Transgenic Mice Containing Megabase Human Immunoglobulin Loci Are Opsonic and Protective against Fatal Pseudomonas Sepsis Infect Immun. 2001 Apr;69(4):2223-9 PMID:11254577 |
Note on publication | Describes the generation of a human mAb against 06ad P. aeruginosa LPS, its binding properites and its protective potential. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
