Key features and details | |
Cat. No. | MABL-2308 |
Name | Anti-M-CSF mAbs |
Clone No. | AFD- Celine |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FACS, inhibition, ELISA |
Species Reactivity | Human, Cynomolgus Monkey |
Basic Information | |
Specificity | This antibody is specific for human M-CSF. It has been shown to cross-react with cynomolgus monkey M-CSF. M-CSF is a secreted cytokine that influences hematopoietic stem cells to differentiate into macrophages or other related cell types. |
Alternative Name | MCSF; CSF1; Macrophage colony-stimulating factor; Macrophage colony- stimulating factor 1; Lanimostim; Proteoglycan macrophage colony-stimulating factor 1; PG-M-CSF 1 |
UniProt | P09603 |
Immunogen | The original antibody was selected from a commercially available phage display library. The library was panned against the receptor-binding domain of human M-CSF (rbdM-CSF). |
Application Notes | The original version of this antibody (human IgG1) was tested for its specificity for human M-CSF and cross-reactivity for cynomolgus M-CSF using ELISA. This antibody binds M-CSF expressed on cells, as shown in a FACS assay that exhibited an EC50 value of 9.7 nM and 13 nM against M-CSF- expressing transfected CHO cells and MDA-MB 231 cells, respectively. This antibody successfully inhibited human and cynomolgus M-CSF binding to recombinant human M-CSF receptor with an IC50 of 4.7 pM and 6.9 pM, respectively. This antibody successfully inhibited the proliferation of M-NFS-60 cells induced by recombinant and native human and cynomolgus M-CSF with an IC50 of 2.7 pM and 23.8 pM for recombinant and native human M-CSF, respectively, and 3.0 pM and 72.4 pM for recombinant and native cynomolgus M- CSF, respectively (US10081675B2). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
