Key features and details | |
Cat. No. | MABL-2340 |
Name | Anti-Melanin mAbs |
Clone No. | AFD- 8C3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | immunotherapy, radioimmunotherapy, ELISA, F, IHC |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody binds melanin. Melanin is a natural pigment found in most organisms is produced in a specialized group of cells called the melanocytes. |
Alternative Name | CHEBI:89634 |
UniProt | |
Immunogen | The original antibody was generated by immunizing female BALB/c mice with melanin particles extracted from Paracoccidioides brasiliensis L-DOPA-induced yeast cells suspended in a 1:1 (vol/vol) emulsion of incomplete Freund adjuvant. |
Application Notes | Paracoccidioides brasiliensis is the agent of the human systemic disease paracoccidioidomycosis. The binding characterization of this antibody towards melanin from various organisms was done using ELISA. This antibody was also used in the immunofluorescence analysis of P. brasiliensis melanized yeast cells (with l-DOPA induction) and wild-type conidia (PMID: 21813659). 213Bismuth and 177Lutetium labelled humanized 8C3 antibody was used in the radioimmunotherapy (RIT) of experimental B16-F10 melanoma (PMID: 31323785). It was demonstrated that a combination of humanized anti-melanin antibody conjugated to 213Bismuth and anti-PD-1 ICB reduced tumor growth and increased survival in the Cloudman S91 murine melanoma DBA/2 mouse model (PMID: 31991626). |
Antibody First Published | Urán et al. Detection of antibodies against Paracoccidioides brasiliensis melanin in in vitro and in vivo studies during infection Clin Vaccine Immunol. 2011 Oct;18(10):1680-8. PMID:21813659 |
Note on publication | Describes the generation and characterization of antibodies towards melanin particles from P. brasiliensis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
