Key features and details | |
Cat. No. | MABL-2366 |
Name | Anti-MHC I (RT1A) mAbs |
Clone No. | AFD- OX-18 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, ELISA, FC, IHC-Fr |
Species Reactivity | Rat |
Basic Information | |
Specificity | OX18 binds to a non-polymorphic / monomorphic determinant of rat MHC class I protein. |
Alternative Name | RT-1A; RT-1A histocompatibility antigen; non-polymorphic determinant of rat MHC class I; MHC class I; OX18; MRC OX18; MRC OX-18 |
UniProt | P16391 |
Immunogen | OX-18 was prepared by immunizing BALB/c mice with rat spleen glycoproteins of the size of Ia antigens which has been depleted of Ia antigens using an OX-6 affinity column. |
Application Notes | This antibody binds to a monomorphic determinant of rat MHC Class I (RT1A). It is routinely used in flow-cytometry applications and has been shown to work in frozen immunohistochemistry assays. |
Antibody First Published | Fukumoto T, McMaster WR, Williams AF Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus. Eur J Immunol. 1982 Mar;12(3):237-43 PMID:6178598 |
Note on publication | Describes the generation of the original hybridoma and the use of OX18 in analyzing RT-1A antigens on rat thymocytes |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
