Key features and details | |
Cat. No. | MABL-2399 |
Name | Anti-MRP1 mAbs |
Clone No. | AFD- A5 scFv |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | A5 scFv binds specifically to the extracellular N-terminus of MRP1, with no cross-reactivity with other MRPs or BCRP. FC analysis showed that A5 reacts strongly with viable ovarian carcinoma 2008/MRP1 and small cell lung carcinoma GLC4/ADR cells that overexpress MRP1. MRP1 is a member of the ABC superfamily of transporter proteins and confers resistance to various toxic compounds and are involved in multi-drug resisitance in chemotherapy. This protein mediates the export of organic anions and drugs from the cytoplasm. |
Alternative Name | LTC4 transporter; Multidrug resistance-associated protein 1; MRP-1; MRPI; MRP-I; ATP-binding cassette sub-family C member 1; Leukotriene C(4) transporter; LTC4 transporter |
UniProt | P33527 |
Immunogen | A5 scFv was isolated from a phage display. The source of the antibody genes came from the spleens of mice which had been immunized with f88 filamentous bacteriophages displaying a peptide derived from the extracellular N-terminus (aa 1-18) of human MRP1. The scFv A5 was expressed in BL21 E. coli cells and purified from the periplasmic fraction by metal-ion affinity chromatography. |
Application Notes | standard and saturation ELISA. The avidity of the scFv wsa increased for FC studies through tetramerization of A5 by adding a BirA tag to the C terminus for site-specific biotinylation and tetramerized using PE-labelled streptavidin. |
Antibody First Published | Binyamin et al. Targeting an extracellular epitope of the human multidrug resistance protein 1 (MRP1) in malignant cells with a novel recombinant single chain Fv antibody. Int J Cancer. 2004 Jul 20;110(6)；882-90. PMID：15170671 |
Note on publication | Describes the generation and characterization of A5 scFv, which binds specifically to MRP1. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
