Key features and details | |
Cat. No. | MABL-2403 |
Name | Anti-MSCP mAbs |
Clone No. | AFD- LC007 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, FC |
Species Reactivity | Monkey, Human |
Basic Information | |
Specificity | This antibody is specific for repeat 14 of MSCP or for an epitope that is only reconstituted when repeat 14 is present. It also binds to cynomolgus MSCP. MSCP is a mitochondrial iron transporter that specifically mediates iron uptake in developing erythroid cells, thereby playing an essential role in heme biosynthesis. The iron delivered into the mitochondria, presumably as Fe2+, is then probably delivered to ferrochelatase to catalyze Fe2+ incorporation into protoprophyrin IX to make heme (By similarity). |
Alternative Name | Mitoferrin-1; SLC25A37; Mitochondrial iron transporter 1; Mitochondrial solute carrier protein; Solute carrier family 25 member 37 |
UniProt | Q9NYZ2 |
Immunogen | The original mouse antibody was raised by immunizing Balb/c mice with a synthetic peptide corresponding to aa 2177-2221 of the human MCSP sequence coupled to KLH. |
Application Notes | The binding epitope of this antibody was mapped by preforming flow cytometry on 4 different constructs. These were: construct 1 containing CSPG repeat 15, construct 2 containing CSPG repeat 14-15, construct 3 containing CSPG repeat 13-15, and construct 4 containing CSPG repeat 12-15 (US2014242083). Flow cytomtery was used to determine whether the human version of this antibody has cross-reactivity with human and cynomolgus antigen. The cells used were HEK-EBNA expressing either a construct containing the C-terminal part of the cynomolgus MCSP protein, a signal peptide for secretion and a N-terminal FLAG-tag or the human variant of this construct (US2014242083). Further, lysis of Colo38 human malignant melanoma cells by human lymphocytes was assessed using the glycoengineerd mouse version of this antibody (US2014242083). |
Antibody First Published | Rataj et al. High-affinity CD16-polymorphism and Fc-engineered antibodies enable activity of CD16-chimeric antigen receptor-modified T cells for cancer therapy Br J Cancer. 2019 Jan;120(1):79-87. PMID:30429531 |
Note on publication | Describes the generation and characterization of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
