Key features and details | |
Cat. No. | MABL-2407 |
Name | Anti-mSOD1 mAbs |
Clone No. | AFD-Nb61 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, IF |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | The original antibody exhibits selective reactivity for misfolded SOD1 proteins (SOD1 G93A, SODox and SOD1 A4V). |
Alternative Name | SOD1 G93A; SODox; SOD1 A4V |
UniProt | |
Immunogen | The original antibody was generated by immunizing llamas simultaneously with recombinant SOD1 WT, SOD1 G93A, and an oxidized form of SOD1 (SODox), followed by library construction. The antibody was isolated by panning against SODox and counterscreened to exclude clones that exhibit high reactivity for the native SOD1 WT protein. |
Application Notes | The specificity of the original format of the antibody was confirmed by ELISA analysis, the antibody exhibited selectivity for human mutant SOD1 (SOD1 G93A, SODox and SOD1 A4V) over murine WT SOD1. The original format of the antibody enhanced SOD1-myc signal intensities in HEK293T cells. The original format of the antibody did not reduce expression levels of misfolded SOD1 protein in mammalian cells, but appeared to stabilize the misfolded conformation of mutant SOD1 in cells and in vitro. As measured by differential scanning fluorimetry, the complex formation between the original format of the antibody and SOD1 A4V have a stabilizing effect on the mutant protein. |
Antibody First Published | Kumar et al. Anti-SOD1 Nanobodies That Stabilize Misfolded SOD1 Proteins Also Promote Neurite Outgrowth in Mutant SOD1 Human Neurons Int J Mol Sci. 2022 Dec 16;23(24):16013. doi: 10.3390/ijms232416013 PMID:36555655 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
