Key features and details | |
Cat. No. | MABL-2413 |
Name | Anti-MUC-1 mAbs |
Clone No. | AFD- VU-3D1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for the SAPDTRPAP epitope of MUC-1. |
Alternative Name | PEM; EMA; PUM; KL-6; PEMT; CA 15-3; H23AG; Mucin-1; Episialin; Tumor- associated mucin; Polymorphic epithelial mucin; Peanut-reactive urinary mucin; Cancer antigen 15-3; Carcinoma-associated mucin; Breast carcinoma-associated antigen DF3; Tumor-associated epithelial membrane antigen |
UniProt | P15941 |
Immunogen | The original antibody was raised by immunizing a mouse with the ZR-75-1 breast cancer cell line. |
Application Notes | The original antibody was used for an ELISA on different peptides of MUC-1 both glycosylated and unglycosylated, here they determined the epitope of the antibody (Karsten et al., 2004; PMID:15115750, Matsushita et al., 2014; PMID:24246952). |
Antibody First Published | Karsten et al. Binding patterns of DTR-specific antibodies reveal a glycosylation-conditioned tumor-specific epitope of the epithelial mucin (MUC1) Glycobiology. 2004 Aug;14(8):681-92. PMID:15115750 |
Note on publication | In the original paper the influence of glycosylation of the immunodominant DTR motif of MUC1 on its antigenicity was determined. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
