Key features and details | |
Cat. No. | MABL-2430 |
Name | Anti-Myc mAbs |
Clone No. | AFD-33 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, IHC |
Species Reactivity | Avian, Human, Mouse |
Basic Information | |
Specificity | This antibody recognizes the human proto-oncogene c-myc, a growth factor which is overexpressed in cancer cells. |
Alternative Name | Transcription factor p64; Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; Proto-oncogene c-Myc; C-33 |
UniProt | P01106 |
Immunogen | The original mouse IgG1 version of this antibody was raised by immunizing mice against the full-length human c-myc protein. The spleen cells of these mice were then fused with mouse myeloma. |
Application Notes | This original mouse IgG1 can be used to detect the human myc-c growth factor. The specificity of this antibody has been confirmed using immunoprecipitation and western blotting (Zhang et al., 2008; PMID: 18438430). In addition, one group used a western blot assay to research the myc-c levels in cells at different stages of cell growth (Shichiri et al., 1993; PMID: 8494788). Another group used western blotting to visualize the amount of myc-c in different tumours (Marin et al., 1995; PMID: 7698223). A final group used immunohistochemistry to analyze the role myc-c plays in tumour development (Banerjee et al., 1996; PMID: 8631582). |
Antibody First Published | Shichiri et al. Effects of c-myc expression on proliferation, quiescence, and the G0 to G1 transition in nontransformed cells Cell Growth Differ. 1993 Feb;4(2):93-104 PMID:8494788 |
Note on publication | Is the first mention of the original mouse IgG1 antibody and describes its usuage |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
