Anti-Myc mAbs

Cat. No.

MABL-2430

Name

Anti-Myc mAbs

Clone No.

AFD-33

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB, IHC

Species Reactivity

Avian, Human, Mouse

Basic Information

Specificity

This antibody recognizes the human   proto-oncogene c-myc, a growth factor which is overexpressed in cancer cells.

Alternative Name

Transcription factor p64; Myc proto-oncogene   protein; Class E basic helix-loop-helix protein 39; Proto-oncogene c-Myc;  C-33

UniProt

P01106

Immunogen

The original mouse IgG1 version of this   antibody was raised by immunizing mice against the full-length human c-myc   protein. The spleen cells of these mice were then fused with mouse myeloma.

Application Notes

This original mouse IgG1 can be used to  detect the human myc-c growth factor. The specificity of this antibody has   been confirmed using immunoprecipitation and western blotting (Zhang et al.,   2008; PMID: 18438430). In addition, one group used a western blot assay to   research the myc-c levels in cells at different stages of cell growth   (Shichiri et al., 1993; PMID: 8494788). Another group used western blotting   to visualize the amount of myc-c in different tumours (Marin et al., 1995;   PMID: 7698223). A final group used immunohistochemistry to analyze the role   myc-c plays in tumour development (Banerjee et al., 1996; PMID: 8631582).

Antibody First   Published

Shichiri et al. Effects of c-myc expression   on proliferation, quiescence, and the G0 to G1 transition in nontransformed   cells Cell Growth Differ. 1993 Feb;4(2):93-104 PMID:8494788

Note on publication

Is the first mention of the original mouse   IgG1 antibody and describes its usuage

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.