Key features and details | |
Cat. No. | MABL-2448 |
Name | Anti-N type Glycophorin A mAbs |
Clone No. | AFD- NNA7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Agglutination reaction, WB, ELISA, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes the human N-type blood-group antigen (MNS blood group) comprised of the N-terminal glycopeptide of glycophorin A (GPA). Glycophorin A is the major sialoglycoproteins and intrinsic membrane protein of the human erythrocyte which bears the antigenic determinants for the MN and Ss blood groups. |
Alternative Name | CD235a; Glycophorin-A; GYPA; GPA; PAS-2; N sialoglycoprotein; Sialoglycoprotein alpha; Glycophorin A N antigen; MNS blood group antigen; glycopeptide N blood-group antigen; red blood cell antigen; N-type glycophorin A; N72 |
UniProt | P02724 |
Immunogen | The original antibody was isolated from light-chain cDNA libraries constructed from mice immunized with N-type glycophorin A. These were inserted into a recombinant pComb3H vector containing the N92 Fd fragment. The N92 Fd fragment: light-chain libraries were panned on N-type glycophorin A or NN red cells. |
Application Notes | This antibody is a synthetic clone derives from the parental clone N72 Fab. This antibody is capable of reacting with N-type glycophorin A and NN red cells in an ELISA and an agglutination assay (PMID: 10220261). This antibody is capable of detecting glycophorin A of N-type and glycophorin B in a western blot assay (PMID: 19833240; 9574545). This antibody was also used in the immunofluorescence staining of transfected CHO cells expressing recombinant N-type GPA (PMID: 14962308). |
Antibody First Published | Czerwinski et al. A molecular approach for isolating high-affinity Fab fragments that are useful in blood group serology. Transfusion. 1999 Apr;39(4):364-71. PMID:10220261 |
Note on publication | This paper describes the use of light chain shuffling to generated mutant parental clones with higher affinity for glycophorin A. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
