Key features and details | |
Cat. No. | MABL-2454 |
Name | Anti-N-ethylmaleimide cysteine mAbs |
Clone No. | AFD- OX-133 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, ELISA, FC |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody recognises cysteine-boud N-ethylmaleimide in a sequence-independent manner. It has no affinity for unmodified peptides or peptides modified with other alkylating agents such as iodoacetamide. |
Alternative Name | NEM cysteine; OX133 |
UniProt | |
Immunogen | Purified MBP-gp120 (HIV-1) reduced using tris(2-carboxyethyl)phosphine (TCEP, 2.5 mM) for 20 minutes and alkylated using NEM (5 mM solubilized in PBS) for 30 minutes. |
Application Notes | OX133 detects NEM-modified proteins and can be used as an affinity matrix to purify NEM-modified proteins from cell lysates. OX133 does not cross react with any other alkylating agent, making it a highly selective reagent for the purification of NEM-labeled proteins and potentially peptides for mass spectrometry-based analysis. |
Antibody First Published | Holbrook et al. OX133, a monoclonal antibody recognizing protein-bound N- ethylmaleimide for the identification of reduced disulfide bonds in proteins MAbs. 2016 May-Jun;8(4):672-7 PMID:26986548 |
Note on publication | Describes the generation and characterisation of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
