Anti-N6-methyladenosine mAbs

Cat. No.

MABL-2456

Name

Anti-N6-methyladenosine mAbs

Clone No.

AFD- 17-3-4-1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

immunoblot, IP

Species Reactivity

Saccharomyces cerevisiae, Human, Mouse

Basic Information

Specificity

This antibody is specific for N(6)-methyladenosine, a modified base found frequently in the mRNA of all   higher eukaryotes and Saccharomyces cerevisiae. It recognises   N(6)-methyladenosine 6A in single-stranded nucleic acids, including both DNA   and RNA.

Alternative Name

m6A; N6-methyladenosine-5'-mono-phosphate

UniProt

Immunogen

This antibody was raised by immunising BALB/c   mice with hapten N6-methyladenosine-5’- monophosphate conjugated to BSA

Application Notes

This antibody has been used in   immunoprecipitation experiments to identify the presence of   N(6)-methyladenosine within mRNA transcripts bound to oligo(dT) magnetic   beads (Bodi et al, 2010). It has been characterised using immunoblot analysis   (Bodi et al, 2010).

Antibody First   Published

Bodi et al. Yeast targets for mRNA   methylation. Nucleic Acids Res. 2010 Sep;38(16):5327-35 PMID:20421205

Note on publication

Describes the original generation and   characterisation of this antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.