Key features and details | |
Cat. No. | MABL-2456 |
Name | Anti-N6-methyladenosine mAbs |
Clone No. | AFD- 17-3-4-1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | immunoblot, IP |
Species Reactivity | Saccharomyces cerevisiae, Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for N(6)-methyladenosine, a modified base found frequently in the mRNA of all higher eukaryotes and Saccharomyces cerevisiae. It recognises N(6)-methyladenosine 6A in single-stranded nucleic acids, including both DNA and RNA. |
Alternative Name | m6A; N6-methyladenosine-5'-mono-phosphate |
UniProt | |
Immunogen | This antibody was raised by immunising BALB/c mice with hapten N6-methyladenosine-5’- monophosphate conjugated to BSA |
Application Notes | This antibody has been used in immunoprecipitation experiments to identify the presence of N(6)-methyladenosine within mRNA transcripts bound to oligo(dT) magnetic beads (Bodi et al, 2010). It has been characterised using immunoblot analysis (Bodi et al, 2010). |
Antibody First Published | Bodi et al. Yeast targets for mRNA methylation. Nucleic Acids Res. 2010 Sep;38(16):5327-35 PMID:20421205 |
Note on publication | Describes the original generation and characterisation of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
