Key features and details | |
Cat. No. | MABL-2461 |
Name | Anti-NC174 mAbs |
Clone No. | AFD- NC10.14 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | CD, FPA, RIA, IF |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody specifically binds the hapten NC174, a trisubstituted guanidinium sweetener. |
Alternative Name | N-(p-cyanophenyl)-N'-(diphenylmethyl)-N''-(carboxymethyl)guanidine; N- (P-CYANOPHENYL)-N'-DIPHENYLMETHYL-GUANIDINE-ACETIC ACID; N-(pcyanophenyl)-N'-(diphenylmethyl)guanidine acetic acid; Trisubstituted guanidine sweetener; TGS |
UniProt | |
Immunogen | The original antibody was generated by murine hybridomas produced by traditional techniques; mice were immunized with TGS covalently conjugated to bovine serum albumin carrier protein through one of the phenyl rings in Freund's complete adjuvant. |
Application Notes | Ligand-induced quenching of IgG NC10.14 was used to determine the dissociation constant of the NC10.14-NC174 complex via IF and Circular Dichroism (CD) Spectroscopy (Viswanathan et al., 1996; PMID: 8756519). This antibody was used to study the ligand specificity of the putative sweet taste receptor binding site. Furthermore, the Fab of this antibody was used to show that aromatic-aromatic contacts at the interdomain surfaces are essential in the origination of CD spectra for both the near- and far- UV region (Tetin et al., 1996; PMID: 8573581). This antibody was examined to determine molecular recognition specificities using 14 different sweetener analogs in a competitive radioimmunoassay (Anchin et al., 1997; PMID: 9587873). |
Antibody First Published | Anchin et al. Variable region sequence and characterization of monoclonal antibodies to a N,N',N"-trisubstituted guanidine high potency sweetener Mol Immunol. 1993 Nov; 30(16):1463-71. doi: 10.1016/0161-5890(93)90108-n PMID:8232332 |
Note on publication | The original publication explores the creation and characterization of a monoclonal antibodies library that targets a trisubstituted guanidinium sweetener. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
