Key features and details | |
Cat. No. | MABL-2478 |
Name | Anti-NKG2A mAbs |
Clone No. | AFD-Z199 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | antagonist, Block, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds the human NKG2a. It can also recognize and bind the CD94/NKG2a heterodimer formed by the covalent association of CD94 with the NKG2a molecule. The CD94/NKG2a heterodimer acts as an inhibitory natural killer cell receptor and binds nonclassical MHC class I protein, HLA-E. NKG2a is found mainly on NK cells and on subsets of CD8+ cells. |
Alternative Name | CD159a; NKG2-A; NKG2a; KLRC1; NK cell receptor A; CD159 antigen-like family member A; NKG2-A/NKG2-B type II integral membrane protein; NKG2-A/B-activating NK receptor |
UniProt | P26715 |
Immunogen | |
Application Notes | This antibody was reported to be a non-competitive antagonist of human CD94/NKG2A receptor expressed on lymphocytes, since it inhibited the function of CD94/NKG2A. Z199 can block the function of human CD94/NKG2A, and specifically induce killing of cells by CD94/NKG2A-restricted lymphocytes in a concentration-dependent fashion (US8796427). This antibody was used to study the covalent interactions between CD94 and NKG2A, which together form the inhibitory natural killer cell receptor (PMID: 9045931). This antibody was used to study the binding interactions of HLA-E (wild-type) tetramer to CD94/NKG2A or CD94/NKG2C expressing cells by flow cytometry (PMID: 14971033). This antibody was used to test the cytotoxic activity of NKL cells against tumor cells. NKL cells were tested for their ability to lyse the indicated targets in a 51Cr release assay, this antibody was found to enhance NK lysis (PMID: 21969815). |
Antibody First Published | Carretero et al. The CD94 and NKG2-A C-type lectins covalently assemble to form a natural killer cell inhibitory receptor for HLA class I molecules. Eur J Immunol. 1997 Feb;27(2):563-7. PMID:9045931 |
Note on publication | Describes the use of this antibody to study the covalent interactions between CD94 and NKG2-A. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
