Key features and details | |
Cat. No. | MABL-2485 |
Name | Anti-NKG2D mAbs |
Clone No. | AFD- KYK-2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | agonist, antagonist, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody bind human NKG2D. Natural killer (NK) cells serve as key innate effectors and their activity has been considered a prognostic biomarker in diverse human diseases. NKG2d functions as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. It provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. |
Alternative Name | KLRK1; CD314; NKG2-D type II integral membrane protein; NK cell receptor D; Killer cell lectin-like receptor subfamily K member 1; NKG2-D-activating NK receptor; KYK2; KYK- 2.0 |
UniProt | P26718 |
Immunogen | The original antibody was isolated from a Fab library generated from the bone marrow from 6 healthy donors by collecting total RNA preparation and RT-PCR amplification of human Vκ, Vλ, and VH encoding sequences. The library was panned against human NKG2D for isolation of this clone. |
Application Notes | The binding of this antibody to NKG2D expressed on subpopulations of human peripheral blood mononuclear cells (PBMC) was done using flow cytometry. This antibody was not capable of binding mouse NKG2D. The IgG1 version of this antibody is reported to show dual antagonistic and agonistic activity. It was reported that soluble KYK-2.0 IgG1 exhibits antagonistic activity through interfering with effector cell to target cell recognition mediated by NKG2D receptor/ligand interactions. KYK-2.0 IgG1 in solution significantly blocked the acquired cytolytic activity of ex vivo expanded human NK cells. The agonistic activity of this antibody was determined using a degranulation assay. It was shown that immobilized KYK-2.0 IgG1 potently induced NK cell degranulation in PBMC from 4 different healthy donors (PMID: 18809410). |
Antibody First Published | Kwong et al. Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity. J Mol Biol. 2008 Dec 31;384(5):1143-56. PMID:18809410 |
Note on publication | Describe the generation, characterization and affinity maturation of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
