Key features and details | |
Cat. No. | MABL-2491 |
Name | Anti-NMDA Receptor mAbs |
Clone No. | AFD-17 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallography |
Species Reactivity | Rat |
Basic Information | |
Specificity | Reacts specifically with rat GluN2B ATD. |
Alternative Name | NMDAR; NMDA-R; Glutamate receptor |
UniProt | Q00960 |
Immunogen | Monoclonal antibodies that bind rat GluN2B ATD were obtained by immunizing mice with the purified intact GluN1-GluN2B NMDA receptors. IgGs were purified from hybridoma cell culture supernatant by Protein-A Sepharose. Fab fragments have been prepared by papain proteolysis followed by re- chromatographing onto Protein-A Sepharose to remove Fc. |
Application Notes | This antibody has been used for the crystallisation of the NMDA receptor in the absence of an allosteric inhibitor at 2.9A (Tajima 2016). |
Antibody First Published | Tajima et al. Activation of NMDA receptors and the mechanism of inhibition by ifenprodil Nature. 2016 May 2; 534(7605): 63–68 PMID:27135925 |
Note on publication | Describes the lack of high resolution structures representing the active state of the NMDA receptor. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
