Key features and details | |
Cat. No. | MABL-2509 |
Name | Anti-Notch 3 mAbs |
Clone No. | AFD- hN3S2-160 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FC; ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human notch 3. |
Alternative Name | Neurogenic locus notch homolog protein 3 |
UniProt | Q9UM47 |
Immunogen | The original antibody was isolated from an scFv phage display library generated from BMV (bone marrow), CS (spleen) and DP47 (frame work based CS) and by panning against a fusion protein containing Notch3 extracellular fragment (amino acid 40-467) and human IgG1-Fc. |
Application Notes | The original human IgG1 antibody hN3S2-160 binds notch 3 with an EC50 =0.06 nM. The binding affinity of this antibody for notch 3 was determined using ELISA. The binding of hN3S2-160 to notch 3 on 293 Flp-TRex cells was done using flow cytometry. This antibody is also capable of blocking notch 3 and DLL4 ligand interaction (WO2010141249). |
Antibody First Published | WO2010141249 |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
