Key features and details | |
Cat. No. | MABL-2518 |
Name | Anti-NS1 mAbs |
Clone No. | AFD- ZKA25 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Zika Virus |
Basic Information | |
Specificity | ZKA25 reacts specifically with ZIKV NS1, shown by ELISA (Stettler et al, 2016). The mAb does not show cross-reactivity with NS1 protein from any of the four DENV serotypes. |
Alternative Name | ZKV NS1; Non-structural protein 1 |
UniProt | |
Immunogen | Antibody ZKA25 was isolated from peripheral blood mononuclear cell (PBMC) and blood plasma collected from a ZIKV-infected patient. |
Application Notes | ZKA25 was shown to react specifically with ZIKV, and not DENV1-4, NS1 protein by standard ELISA (Stettler et al, 2016). This mAb was also used in a sandwich ELISA as a serological diagnostic tool to detect viral NS1 from biological fluids obtained from patients with diagnosed ZIKV and DENV infection. This antibody can be used as a positive cotrol in the detection of Zika virus using the blockade-of- binding assays described by Balmaseda (PMID: 28716913 and 29305550) in combination with antibody ZKA35 (Ab01036-10.0-BOB). |
Antibody First Published | Stettler et al. Specificity, cross-reactivity, and function of antibodies elicited by Zika virus infection. Science. 2016 Aug 19;353(6301):823-6. PMID:27417494 |
Note on publication | Describes the isolation and characterisation of a large panel of human mAbs both targeting ZIKV proteins as well as mAbs cross-reactive with DENV. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
