Anti-Nuclear Transport Factor 2 (NTF2) mAbs

Cat. No.

MABL-2526

Name

Anti-Nuclear Transport Factor 2 (NTF2) mAbs

Clone No.

AFD- 5-E8

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, IF

Species Reactivity

Human

Basic Information

Specificity

The antibody is specific for human and Xenopus NTF2, recognizing an epitope on the N terminus.

Alternative Name

NTF2; NTF-2; Placental protein 15; PP15

UniProt

P61970

Immunogen

The antibody was raised by immunizing BALB/c   mice with a synthetic peptide corresponding to the N-terminal 14 amino acids   (MGDKPIWEQIGSSF) of NTF2.

Application Notes

Western blotting showed this antibody (IgG1 format) detects both human and Xenopus NTF2. Immunofluorescence was performed on BHK21 cells using this antibody, finding NTF2 is a nuclear protein (Steggerda et al, 2000; pmid: 10679025).

Antibody First   Published

Steggerda et al. Monoclonal antibodies to   NTF2 inhibitnuclear protein import by preventing nuclear translocation of the   GTPase Ran. Mol Biol Cell. 11(2):703-19 (2000) PMID:10679025

Note on publication

The article describes the generation and characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.