Key features and details | |
Cat. No. | MABL-2528 |
Name | Anti-Nucleophosmin mAbs |
Clone No. | AFD- NA24 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific towards nucleophasmin which is a widely expressed phosphorylated protein involved in various processes, such as ribosome assebly and transport, cell proliferation, protein chaperoning and centrosome duplication. Aberrations involving nucleophasmin are found in multiple conditions, for instance in a form of non-Hodgkin lymphoma or acute promyelocytic leukemia. |
Alternative Name | NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; B23; NPM1; Nucleolar protein NO38 |
UniProt | P06748 |
Immunogen | This antibody was raised by immunising BALB/C mice with GST fusion protein containing the N-terminal part of nucleophosmin fused to 14 amino acid of ALK protein. |
Application Notes | Mutations associated with nucleophasmin are present in varous conditions and this antibody has proven useful in different experiments investigating their molecular underpinnings. One group used NE24 antibody to perform immunohistochemical staining confirming that anaplastic large-cell lymphoma involves expression of mutated NPM-ALK fusion (Cordell et al., 1999). Finally, alterations of nucleophasmin expression were found in many cancers where this antibody might be utilised to analyse them. For example, Gimenez and colleagues (2010) performed immunohistochemical stainings and Western blot analyses to demonstrate aberrations in nucleophasmin expression in brain cancer. |
Antibody First Published | Cordell et al. Detection of normal and chimeric nucleophosmin in human cells. Blood. 1999 Jan 15;93(2):632-42. PMID:9885226 |
Note on publication | This article describes generation and characterisation of NA24 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
