Key features and details | |
Cat. No. | MABL-2544 |
Name | Anti-Oligomeric α-syn mAbs |
Clone No. | AFD- 10H (syn-10H) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, WB, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes the later-stage oligomeric morphological form of human alpha- synuclein. Alpha-synuclein is a small 140 amino acid presynaptic neuronal protein, a major component of Lewy bodies and a member of the synuclein family. Misfolding, abnormal accumulation, and secretion of α- Synuclein (α-Syn) are closely associated with synucleinopathies, including Parkinson’s disease (PD). α-syn is abundant in the brain, and smaller amounts are found in the heart, muscles, and other tissues. In the brain, α-syn is found mainly at the tips of neurons in the specialized structures known as presynaptic terminals and has been shown to comprise up to ∼1% of total proteins in the neuronal cytosol. |
Alternative Name | SNCA; NACP; PARK1; alpha-syn; α-Synuclein; α-syn; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; oligomeric α-synuclein; oligomeric α- syn |
UniProt | P37840 |
Immunogen | The original antibody was isolated from a human Tomlinson I and J scFv antibody libraries by panning the library against diluted sample of alpha-synuclein (20 uM) containing predominantly larger aggregate forms. |
Application Notes | The binding of this antibody to oligomeric forms of alpha-synuclein was confirmed using ELISA. This antibody is not capable of recognizing monomeric and fibrillar forms of alpha-synuclein. This antibody could recognize the late stage alpha-synuclein aggregates occurring during (10–22 day) time points. It was capable of binding aggregates with molecular masses of 42 and ~80 kDa corresponding to trimeric and hexameric forms in a western blot. The scFv version of this antibody inhibits aggregation of alpha-synuclein in vitro, blocks extracellular alpha-synuclein-induced toxicity in both undifferentiated and differentiated human neuroblastoma cell lines (SH-SY5Y), and specifically recognizes naturally occurring aggregates in Parkinson's disease but not in healthy human brain tissue (PMID: 19141614). A fusion protein comprising D5 scFv, cell pentrating peptide like penetratin were found to be effective in Lewy body disease. |
Antibody First Published | Emadi et al. Detecting Morphologically Distinct Oligomeric Forms of α- Synuclein. J Biol Chem. 2009 Apr 24; 284(17): 11048–11058. PMID:19141614 |
Note on publication | Describes the isolation of antibody fragments that bind to a specific morphology of alpha-synuclein using phage display antibody technology and atomic force microscopy (AFM). |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
