Key features and details | |
Cat. No. | MABL-2565 |
Name | Anti-Oxidized phospholipid mAbs |
Clone No. | AFD- E06 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, RIA, WB, ELISA, FC, IF, IHC |
Species Reactivity | Rabbit, Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for oxidized lipoproteins. |
Alternative Name | OxPL; OxLDL; CuOx-LDL; copper-oxidized low-density lipoproteins; EO6 |
UniProt | |
Immunogen | The original mouse antibody was raised from apoE-deficient mice without any external immunogens. They were fed a high fat diet containing 12.8% milk fat and 0.15% cholesterol for 7 months. Such dietary intervention induces extensive aortic atherosclerosis, and is accompanied by the formation of high titers of E0 antibodies to MDA-lysine. |
Application Notes | To determine the antibody's binding affinity to native or modified human LDL, a radioimmunoassay was conducted using the mouse version of this antibody. Immunocytochemistry was performed on atherosclerotic lesions of LDL receptor-deficient rabbits, balloon-catheterized, cholesterol-fed New Zealand white rabbits, and human brain arteries using the mouse version of this antibody (Palinski et al., 1996; PMID: 8698873). The mouse version of this antibody was characterized through various experiments and assays. An antibody absorption experiment was conducted on increasing concentrations of native PAPC, OxPAPC, or microemulsions made from lipids extracted from native LDL or CuOx-LDL. Additionally, it was used in an ELISA performed on antigens in PBS. In a Western blot assay, the alkaline phosphatase-labeled goat anti-mouse IgM version of this antibody was utilized to detect CuOx-LDL and native LDL. A flow cytometric experiment was performed on resident peritoneal macrophages isolated from female Swiss-Webster mice using the original mouse version of this antibody. |
Antibody First Published | Palinski et al. Cloning of monoclonal autoantibodies to epitopes of oxidized lipoproteins from apolipoprotein E-deficient mice. Demonstration of epitopes of oxidized low density lipoprotein in human plasma J Clin Invest. 1996 Aug 1;98(3):800-14. PMID:8698873 |
Note on publication | The original publication discusses the cloning of monoclonal autoantibodies to epitopes of oxidized lipoproteins from apolipoprotein E-deficient mice ("E0 antibodies"), demonstrating the occurrence of these epitopes in human plasma and atherosclerotic lesions, and highlighting the immunological response to various epitopes of oxidized lipoproteins in vivo. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
