Key features and details | |
Cat. No. | MABL-2576 |
Name | Anti-p130/CAS mAbs |
Clone No. | AFD- 8G4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, IF |
Species Reactivity | Rat, Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for p130/CAS amino acids 576 to 796 and recognizes rat, mouse and human homologs. P130cas is a docking protein which plays a central coordinating role for tyrosine-kinase- based signaling related to cell adhesion (By similarity). Implicated in induction of cell migration and cell branching (By similarity). Involved in the BCAR3-mediated inhibition of TGFB signaling (By similarity). |
Alternative Name | Breast cancer anti-estrogen resistance protein 1; Bcar1; CRK-associated substrate; p130cas |
UniProt | Q63767 |
Immunogen | The original mouse version of this antibody was raised by immunizing a mosue with a GST fusion protein corresponding to residues 576-796 of rat p130 Cas. |
Application Notes | The function of CAS in cell signaling was researched. For this a western blot was preformed on rat and murine fibroblasts, as well as human osteosarcoma, and COS-7 cells using the murine version of this antibody (Bouton and Burnham, 1997; pmid:9388023). Furthermore with the same goal CE cells were immunostained using the mouse version of this antibody (Bouton and Burnham, 1997; pmid:9388023). Immunoblotting was preformed on NIH3T3 cells using the mouse version of this antibody. This was done to research FAK phosphorylation (Lim et al, 2004; pmid:15123632). To assay the effect of PTP-PEST overexpression the mouse version of this antibody was used for immunoblotting on Bosc23 cells transfected with the gene encoding full-length human PTP-PEST (Garton and Tonks, 1999; pmid:9920935). |
Antibody First Published | Bouton and Burnham Detection of distinct pools of the adapter protein p130CAS using a panel of monoclonal antibodies Hybridoma. 1997 Oct;16(5):403-11. PMID:9388023 |
Note on publication | he isolation and characterization of a panel of monoclonal antibodies that specifically recognize CAS was reported. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
