Key features and details | |
Cat. No. | MABL-2580 |
Name | Anti-P3 idiotype mAbs |
Clone No. | AFD-1E 10 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vivo, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is specific for the antigen binding site of the anti-NeuGcGM3 antibody P3 (Ab04722). |
Alternative Name | Mabai 1E10 |
UniProt | |
Immunogen | The original antibody was generated by immunizing a BALB/c mouse with KLH-coupled P3 Mab. |
Application Notes | The original (mouse IgG1) and chimeric version (human IgG1) have been tested in various experimental settings to assess the antibody's antitumor effects. In vivo studies demonstrated significant reductions in the growth of the mammary carcinoma cell line F311 and spontaneous lung metastasis in mice vaccinated with a KLH-coupled version of the original antibody. Additionally, intravenous administration of the original antibody to mice inoculated with B16 melanoma cells resulted in a dramatic reduction in lung metastases compared to irrelevant IgG treatment. In vitro, the binding activity of the original version of the antibody was confirmed through ELISA, where the chimeric version of the antibody specifically bound to MAb P3-coated microtiter plates, while a negative control antibody showed no binding (US8758753B2). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
