Anti-p53 (a.a. 16-25) mAbs

Cat. No.

MABL-2582

Name

Anti-p53 (a.a. 16-25) mAbs

Clone No.

AFD- X77

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB, ELISA, IF

Species Reactivity

Xenopus, Human, Mouse

Basic Information

Specificity

This antibody recognizes the human p73   protein as well as the human and xenopus p53 protein.

Alternative Name

Cellular tumor antigen p53; Antigen   NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

UniProt

P04637

Immunogen

The original mouse IgG1 version of this antibody   was raised by immunizing mice against xenopus p53

Application Notes

This original mouse IgG1 can be used to   detect human p73 as well as human and xenopus p53. The reaction of this   antibody with the two proteins was verified using immunoprecipitation and   western blot (Le Bras et al., 2002; PMID: 11850851). The same group used   indirect immunofluorescence to verify the specificity of the antibody (Le   Bras et al., 2002; PMID: 11850851). Another group used the antibody with   immunoprecipitation and immunoblotting to detect the accumulation of human   p53 with increasing levels of calpastatin (Pariat et al., 1997; PMID:   9111352).

Antibody First   Published

Le Bras et al. Monoclonal antibodies raised   against Xenopus p53 interact with human p73 Oncogene. 2002 Feb   14;21(8):1304-8 PMID:11850851

Note on publication

Describes the characterization of the   original X77 clone which was generated in the same paper

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.