Key features and details | |
Cat. No. | MABL-2591 |
Name | Anti-paclitaxel mAbs |
Clone No. | AFD- 3A3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | |
Basic Information | |
Specificity | This antibody is specific for paclitaxel, an anti-neoplastic agent derived from the Pacific yew. It displays low cross-reactivity with other naturally-occurring taxanes. |
Alternative Name | PT; PTX; Taxol; 7-Xylosylpaclitaxel; Taxol-7-xyloside; 7-xylosyltaxol |
UniProt | |
Immunogen | This antibody was raised by immunising BALB/c mice with a solution of 7-xylosyltaxol conjugated to the carrier protein bovine serum albumin (XylTax–BSA).7-xylosyltaxol is a taxol (paclitaxel) derivative, which binds to tubulin and inhibits microtubule dis |
Application Notes | This antibody has been used in an indirect competitive enzyme-linked immunosorbent assay to determine the paclitaxel content of yew tree samples, with a detection range of 0.098–312.5 μg ml−1 (Chao et al, 2012). An scFv derived from this antibody reacts with both free and immobilised paclitaxel in icELISA analysis, and exhibits less cross-reactivity for paclitaxel-related compounds (Yusakul et al, 2016). |
Antibody First Published | Chao et al. Development of an indirect competitive enzyme-linked immunosorbent assay (icELISA) using highly specific monoclonal antibody against paclitaxel J Nat Med (2013) Volume 67, Issue 3, pp 512–518 PMID:23007175 |
Note on publication | Describes the original generation of this antibody, and its use in an indirect competitive enzyme-linked immunosorbent assay. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
