Anti-Paxillin (LD2 Motif) mAbs

Cat. No.

MABL-2616

Name

Anti-Paxillin (LD2   Motif) mAbs

Clone No.

AFD- LD2 sAB

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA

Species Reactivity

Human

Basic Information

Specificity

The antibody binds specifically to the LD-2 motif of human paxillin with a Kd of 24 nM.

Alternative Name

PXN

UniProt

P49023

Immunogen

Peptide corresponding to paxillin-LD2 motif (residues   141-156) fused to MBP; peptide sequence: NLSELDRLLLELNAVQHNP.

Application Notes

This antibody binds to the LD2-motif of  human paxillin, a signal transduction adaptor protein involved in focal   adhesion, with binding partners including integrins, Src, FAK or actin complexes. The antibody inhibits complex formation between paxillin and FAK.   Paxillin is involved in both inside-out cell signalling and outside-to-inside   cell signalling.

Antibody First   Published

Nocula-Lugowska et al. Engineering Synthetic Antibody Inhibitors Specific for LD2 or LD4 Motifs of Paxillin. Journal of  Molecular Biology 2015; S0022-2836(15)00344-7 PMID:26087144

Note on publication

Describes the generation and crystallization of two synthetic antibodies against the LD2 and LD4 motifs of human paxillin

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.