Key features and details | |
Cat. No. | MABL-2645 |
Name | Anti-PD-1H mAbs |
Clone No. | AFD- MH5A |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | therapeutic, Block, ELISA, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody specifically binds PD-1H. |
Alternative Name | VISTA; VISR; B7-H5; B7H5; GI24; PP2135; SISP1; DD1alpha; C10orf54; chromosome 10 open reading frame 54; V-set immunoregulatory receptor |
UniProt | Q9D659 |
Immunogen | The antibody was generated by immunising Armenian hamsters with mPD-1HIg. |
Application Notes | The antibody has been labelled with biotin and used to stain formalin-fixed tissues from wild-type C57BL/6 mice at 6 weeks of age. This showed specific staining for PD-1H in the T cell zone and marginal zone. The antibody stains P815 cells transfected with full-length PD-1H plasmid, but not control plasmid. Antibody specificity has been confirmed by ELISA. A single 200-μg dose of MH5A prevented aGVHD in mouse models, caused a reduction in accumulation and expansion of CD8+ and CD4+ T cells in spleens and livers and greatly reduced numbers of inflitrating T cells in all aGVHD target tissues examined. Few mAbs targeting a single cosignalling molecule have such a potent preventative effect (Flies D et al, 2011). |
Antibody First Published | Flies D et al. Cutting Edge: A Monoclonal Antibody Specific for the Programmed Death-1 Homolog Prevents Graft-versus-Host Disease in Mouse Models J Immunol August 15, 2011, 187 (4) 1537-1541 PMID:21768399 |
Note on publication | Describes the generation of the antibody and characterisation of its theraputic function in models of graft-versus-host disease. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
