Anti-PD-L1 mAbs

Cat. No.

MABL-2647

Name

Anti-PD-L1 mAbs

Clone No.

AFD- 29E.2A3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, FC, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody binds human PD-L1.

Alternative Name

CD274; PDL1; B7-H1; B7H1; B7 homolog 1; hPD-L1; PDCD1L1; PDCD1LG1; PDCD1 ligand 1; Programmed cell death 1 ligand 1;   Programmed death ligand 1

UniProt

Q9NZQ7

Immunogen

Application Notes

The original format of the antibody was able   to block the interaction between PD-L1 on the 300.19-hPD-L1 cells and B7-1 on   the plate and the interaction between PD-L1 on the 300.19-hPD-L1 cells and   PD-1 on the plate (Butte et al., 2008; PMID: 18585785). This antibody was   used for detection of PD-L1 expressed in 293T cells by flow cytometry (Broos   et al. 2019, PMID: 31394834). The humanized version of the antibody was   constructed (AU2019201688). Immunohistochemical staining of frozen tissue   sections was performed using the antibody to stain thymic epithelia (Brown et   al., 2003; PMID: 12538684).

Antibody First   Published

Latchman et al. PD-L2 is a second ligand for   PD-1 and inhibits T cell activation Nat Immunol. 2001 Mar;2(3):261-8. doi: 10.1038/85330. PMID:11224527

Note on publication

The original paper describes the generation   and characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.