Anti-PD-L1 mAbs

Cat. No.

MABL-2650

Name

Anti-PD-L1 mAbs

Clone No.

AFD- AB1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, FC

Species Reactivity

Human

Basic Information

Specificity

This antibody binds human PD-L1.

Alternative Name

CD274; PDL1; B7-H1; B7H1; B7 homolog 1; hPD-L1; PDCD1L1; PDCD1LG1; PDCD1 ligand 1; Programmed cell death 1 ligand 1;   Programmed death ligand 1

UniProt

Q9NZQ7

Immunogen

The original antibody was generated from a yeast   display library by panning against B7-H1-Fc biotinylated protein.

Application Notes

The scFv version of this antibody binds   human B7-H1 with an affinity of 2.88 nM. The ability of this antibody to   block binding of PD-1-Fc protein or B7.1-Fc protein to B7-H1-Fc was evaluated   using ELISA. This antibody could effectively block B7-H1/PD-1 binding and   B7-H1/B7.1 binding with an IC50 value of 1.37µg/ml and 0.521.37µg/ml   respectively. The ability of the AB1 to bind to human B7-H1, mouse B7-H1 was   determined by flow cytometric analysis. This antibody did not exhibit any   binding to 293F cells transfected with mouse B7-H1, human PD-1 or human ICOS.   This antibody was also shown to IFN-γ release in a PBMC asssay. It was also   reported that this antibody can inhibit A375 human melanoma tumor growth in   mouse model (WO2015195163).

Antibody First   Published

WO2015195163

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.