Anti-PD-L1 mAbs

Cat. No.

MABL-2653

Name

Anti-PD-L1 mAbs

Clone No.

AFD- BT613

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, in vitro, in vivo, inhibition, SPR  

Species Reactivity

Human, Mouse

Basic Information

Specificity

The original antibody is specific for PD-L1.   The antibody recognises human and mouse PD-L1.

Alternative Name

CD274; B7-H1; B7 homolog 1; PDCD1 ligand 1; Programmed death ligand 1; hPD-L1; Programmed cell death 1 ligand 1

UniProt

Q9NZQ7

Immunogen

The original antibody was generated by   immunizing transgenic mice with PD-L1, followed by antibody library   construction and phage display-based selection against PD-L1.

Application Notes

The binding affinity of the antibody to   PD-L1 was measured by surface plasmon resonance. The binding affinity of the   antibody was measured on the cell lines with high expression of PD-L1 by   FACS. The specificity of the original format of the antibody was confirmed by   ELISA analysis. The antibody had good activity in blocking the binding of   PD-L1 to PD-1 and PD-L1 to B7-1 is shown by ELISA analysis. The antibody   could block the binding of PD-1 and PD-L1 at the cellular level in in vitro   experiments. The antibody was used for in vivo experiments on colon cancer   tumor model. B-hPD-1 mice with human PD- 1 and MC38-hPD-L1 colon cancer cells   with human PD-L1 were treated with the antibody. Results showed inhibition of   tumor volume growth in mice treated with the antibody.

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.