Anti-PD-L1 mAbs

Cat. No.

MABL-2656

Name

Anti-PD-L1 mAbs

Clone No.

AFD- JC071

From

Recombinant Antibody

Isotype

Engineer antibody

Application

FC, WB, ELISA, Block

Species Reactivity

Dog

Basic Information

Specificity

This antibody binds canine PD-L1.

Alternative Name

CD274; Programmed death ligand 1; PDL-1; PDL1

UniProt

E2RKZ5

Immunogen

The original antibody was generated by immunizing BALB/c mice with recombinant canine PD-L1 using an exponentially increasing dosing strategy with MPLA as adjuvant.

Application Notes

This antibody is recommended for detection   of canine PD-L1. This antibody successfully detected canine PD-L1 in a   western blot assay. JC071 was used to determine the increased PD- 1   expression on canine PBMCs stimulated with peptidoglycan or  lipopolysaccharide using flow cytometry. JC071 was also able to detect   recombinant PD-L1 expressed on DH82 cell line both with and without in vitro   activation using IFN-γ in flow cytometry. It also recognized recombinant   canine PD-L1 expressed on CHO cells in flow cytometry. The ability of the   antibody to block interactions between PD-1 and PD-L1 was checked using   ELISA. It is known that blockade of PD-L1 and PD-1 interactions causes   increase in Ifn-γ level. JC071 successfully blocked PD-L1 and amplified IFN-γ   production in culture by approximately 6-fold. Thus, JC071 alleviated T-cell   suppression and led to enhanced functional activity of T cells in culture and   have potential application as an immunotherapy reagent for cancer treatment   (PMID: 32614928).

Antibody First   Published

Choi et al. Development of canine PD-1/PD-L1 specific monoclonal antibodies and amplification of canine T cell function.   PLoS One. (2020); 15(7): e0235518 PMID:32614928

Note on publication

Describes the generation and   characterization of antibodies towards canine PD-1 and PD-L1 for their   potential use in therapeutics for veterinary oncology.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.