Key features and details | |
Cat. No. | MABL-2666 |
Name | Anti-PDL2 mAbs |
Clone No. | AFD-HZ-D-Na-96-1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Block, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human PD-L2, expressed on dendritic cells, IFN-gamma-treated monocytes, placental endothelium and medullary thymic epithelial cells. This protein is an immune checkpoint receptor ligand which plays a role in negative regulation of adaptive immune response. It is involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. |
Alternative Name | CD273; PD-L2; PD L2; PDCD1LG2; PDCD1L2; B7DC; B7-DC; PD-1 ligand 2; PDCD1 ligand 2; PDCD1 ligand 2; Programmed cell death 1 ligand 2; Programmed death ligand 2; Butyrophilin B7-DC |
UniProt | Q9BQ51 |
Immunogen | The original antibody was generated by immunizing 2 alpacas with human PDL2 antigen in Freund's adjuvant. The peripheral blood of the alpaca was taken to generate a yeast display nanobody library and the particular clone was isolated by screening the library against human PDL2. Later on the single domain antibody was humanized using framework grafting method. |
Application Notes | This antibody binds human PD-L2 with a binding affinity of Kd= 1.68 nM. The binding ability of this antibody towards human PD-L2 expressed on the surface of CHO cells was determined using flow cytometry. This antibody is capable of blocking the interaction between PDL2 and PD-1. This antibody in combination with an anti-PDL1 antibody 14-02-3 was used in the generation of a bispecific antibody targeting human PDL1 and PDL2 simultaneously. The resulting bispecific antibody was capable of blocking binding of PD-L2/PD-L1 to PD-1 and also block PDL1/PDL2/PD1/luc signaling pathway (WO2021197358). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
