Key features and details | |
Cat. No. | MABL-2669 |
Name | Anti-pectinase mAbs |
Clone No. | AFD- B1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assays, WB, ELISA |
Species Reactivity | Fungal |
Basic Information | |
Specificity | This antibody binds to pectinases from various pathogenic fungi from such groups as ascomycetes, basidiomycetes, and oomycete. |
Alternative Name | Polygalacturonase; PG; Endopolygalacturonase; ScFvB1; AY741402; enzyme pectinase |
UniProt | |
Immunogen | Spleen mRNA from mice immunized with extracellular proteins from Rhizoctonia solani was used to construct a cDNA library from which antipectinase ScFv antibodies were isolated using phage display techniques (Manatunga et al., 2005). |
Application Notes | B1 scFV antibody was shown to inhibit polygalacturonase (PG) activity in a number of pathogenic species of fungi and thus, it is a highly promising agent in the research on the methods of plant fungal diseases prevention (Manatunga et al., 2005). Furthermore, this antibody was also able to inhibit maceration caused by infection of plant tissue by necrotrophic fungi (Manatunga et al., 2005). |
Antibody First Published | Manatunga et al. Maceration of plant tissue by fungi is inhibited by recombinant antipectinaseantibodies European Journal of Plant Pathology (2005) 112: 211–220 |
Note on publication | This article describes the generation and characterisation of the B1 scFv. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
