Key features and details | |
Cat. No. | MABL-2672 |
Name | Anti-Pep27 mAbs |
Clone No. | AFD- F9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IB, ELISA |
Species Reactivity | Streptococcus pneumoniae (S. Pneumoniae) |
Basic Information | |
Specificity | This antibody is specific for the Streptococcus pneumoniae Pep27. The epitope recognized by this antibody is located within the residues 18-27, with key residues crucial for the binding being Y24, R26, and K27, as identified through alanine scanning and further supported by molecular docking analyses. Streptococcus pneumoniae Pep27 is a small, secreted peptide consisting of 27 amino acids initially described as a "death signal peptide". |
Alternative Name | Pep27 protein |
UniProt | B2IMR9 |
Immunogen | The original antibody was selected from a synthetic human scFv library encoding HA-tagged scFv clones through five rounds of biopanning against synthetic and recombinant Pep27. |
Application Notes | The dissociation constant (Kd) of the original human scFv version of this antibody was 0.5 µM as measured in an ELISA. This antibody specifically detected Pep27 in an immunoblot and in an environment mimicking in vivo conditions, demonstrated in human serum (Kim et al. 2018; PMID: 29753735). This antibody was successfully converted and expressed as a Fab, scFv-Fc, scFv-CH, and IgG using an antibody reformatting vector system (AbVec) (Kim, Park, & Lee, 2019). |
Antibody First Published | Kim et al., Molecular characterization of single-chain antibody variable fragments (scFv) specific to Pep27 from Streptococcus pneumoniae. Biochem Biophys Res Commun. 2018 Jun 27;501(3):718-723. doi: 10.1016/j.bbrc.2018.05.054. PMID:29753735 |
Note on publication | The original publication describes the development and characterization of two monoclonal scFvs that are specific to Streptococcus pneumoniae Pep27. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
