Anti-Phosphatidylinositol-3,4-diphosphate mAbs

Cat. No.

MABL-2685

Name

Anti-Phosphatidylinositol-3,4-diphosphate  mAbs

Clone No.

AFD- 8C2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IB, Liposome lysis assay, IF

Species Reactivity

Human

Basic Information

Specificity

8C2 does not bind PI-4,5-P2, PI-3,4-P2, or   PI-4-P2 and is specific towards PI-3,4-P2. It also does not bind PC, PE, PS,   PA, PI.

Alternative Name

PtdIns(3,4)P2; PI-3,4-P2; PIP2

UniProt

Immunogen

Salmonella minnesota cells were coated with   10 μg of PI 3,4‐P2 and injected into   mice. Hybridomas were formed by fusion with SP2/0 cells, and the hybridoma supernatants screened for antibodies against PI 3,4‐P2 by a liposome lysis assay.

Application Notes

Liposome lysis assays established that 8C2 does not bind to PI-4-P, PI-4,5-P2 or PIP3 molecules. This was also confirmed  by a dot-blot assay. 8C2 was used to stain 293T cells for PI-3,4-P2 with a   second antibody conjugated with FITC.

Antibody First   Published

Yokogawa et al, 2000. Evidence that 3'-   phosphorylated polyphosphoinositides are generated at the nuclear surface:   use of immunostaining technique with monoclonal antibodies specific for PI   3,4-P(2). FEBS Lett. 2000 May 12;473(2):222-6. PMID:10812079

Note on publication

Describes the original generation of the antibody, its characterisation through liposome lysis assays, and its   application to cell staining.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.