Anti-Phospho-IRF5 mAbs

Cat. No.

MABL-2687

Name

Anti-Phospho-IRF5 mAbs

Clone No.

AFD- AbAb01-IRF5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

immunoblot, ELISA, FC

Species Reactivity

Human, Mouse

Basic Information

Specificity

This antibody binds human and mouse IRF5   phosphorylated at serine 446. IRF5 is a transcription factor that plays a   critical role in innate immunity by activating expression of type I   interferon (IFN) IFNA and INFB and inflammatory cytokines downstream of   endolysosomal toll-like receptors TLR7, TLR8 and TLR9.

Alternative Name

pS446-type IRF5; phosphorylated IRF5; IRF5;   IRF-5; Interferon regulatory factor 5

UniProt

Q13568

Immunogen

The original antibody was generated by immunizing   rabbit with a synthetic peptide comprising amino acid sequence   'SIRLQI(pS)NPDLKDC'. The immunogen sequence was phosphorylated at the 7th   serine residue which corresponds to serine 446 of human IRF5. A phage display   scFv library was generated using the cells from the spleen and the specific   antibody was isolated by positive screening against biotinylated   'SIRLQI(pS)NPDLKDC' peptide and negative selection biotinylated   non-phosphorylated peptide 'SIRLQISNPDLKDK'.

Application Notes

The binding evaluation of this antibody to human and mouse pS446-type IRF5 was done using ELISA. This antibody was used   in the detection of endogenous IRF5 upon TLR stimulation of human immune cell   lines. Phosphorylation of endogenous IRF5 in CAL-1 cells stimulated with   R-848, a ligand for TLR7 and TLR8, was analyzed by immunoblotting. Similar   method was used for the detection of endogenous IRF5 phosphorylation upon TLR   stimulation of human PBMCs. This antibody was also used for the determination   of phosphorylated IRF5 positive cell population in human PBMC using flow   cytometry. The antibody was also able to detect phosphorylated mouse IRF5   protein. This antibody was also used in the phosphorylated IRF5 from splenic   CD11c cells of 18-20 week old female Lyn-deficient mice and NZB/W F1 mice, a   widely used SLE model mouse.

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.