Anti-procollagen mAbs

Cat. No.

MABL-2748

Name

Anti-procollagen mAbs

Clone No.

AFD- 1E8.33

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, IHC

Species Reactivity

Human

Basic Information

Specificity

The antibody is specific for human   procollagen 11A1. The epitope recognized is encompassed in the   YNYGTMESYQTEAPR amino acid stretch within the variable region of human   procollagen 11A1. The antibody does not cross-react with human procollagen   5A1.

Alternative Name

DMTX1/1E8.33; human procollagen 11A1; Type   V/XI procollagen alpha 1 chain

UniProt

Q4AE66

Immunogen

The original antibody was generated by   immunizing BALB/c mice against the variable region of human procollagen   COL11A1.

Application Notes

The original format of the antibody detected   human procollagen 11A1 on A204 cells by western blot analysis. The original   format of the antibody showed a strong cytoplasmic granular staining on A204   cells and a weaker staining on SAOS-2 cells by immunohistochemistry. The   original format of the antibody showed a strong cytoplasmic staining of   peritumoral myofibroblasts (García-Ocaña et al., 2012; PMID: 22322826).

Antibody First   Published

García-Ocaña et al., Characterization of a   novel mouse monoclonal antibody, clone 1E8.33, highly specific for human   procollagen 11A1, a tumor-associated stromal component. Int J Oncol. 2012   May;40(5):1447-54. doi: 10.3892/ijo.2012.1360. Epub 2012 Feb 7. PMID:22322826

Note on publication

The original paper describes the generation   and characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.