Key features and details | |
Cat. No. | MABL-3014 |
Name | Anti-TCR mAbs |
Clone No. | AFD- BMA031 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FACS, IP, Block, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells. TCR-αβ is expressed by the majority of mature CD3+ T cells. |
Alternative Name | Human α/β T Cell Receptor (TCR) |
UniProt | |
Immunogen | The original antibody was generated by immunizing mice with human perihperal blood T- lymphocytes. |
Application Notes | This antibody is recomended for detection of viable human αβ+ TCR lymphocyte. It is highly effective in modulating immune reactions and is capable of activating human T lymphocytes in vito (PMID: 3092794). Double immunofluorescence staining was used to ascertain that this antibody does not cross react with γδ+ TCR cells. FACS analysis confirmed that BMA031 did not react with γδ+ TCR cells. BMA031 successfully precipitates TCR αβ/CDR complex (PMID: 1704361). BMA031 in conjugation with triple drug induction treatment in kidney-transplanted patients showed no side effects and reduction in graft rejections (PMID: 2978369). A clinical phase II trial administered 50 mg of murine anti-human alpha/beta- TCR mAb (BMA 031) intravenously on the day of, as well as 2 and 4 days after, cadaveric donor renal transplantation in combination with a CsA/prednisone regimen. |
Antibody First Published | Kurrle et al. Distinction of two CD3-monoclonal antibodies in respect to the effectivity to modulate the induction of IgM- and gamma-interferon synthesis. Behring Institute Mitteilungen (1986), (80):48-58. PMID:3092794 |
Note on publication | Describes the role of this antibody as a tool for analysing mechanisms of T cell activation, interaction between T-cells, B-cells, accessory cells and intermediating immune factors. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
