Key features and details | |
Cat. No. | MABL-3030 |
Name | Anti-Testosterone mAbs |
Clone No. | AFD- 5F2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | The antibody is specific for the testosterone molecule. Testosterone is the primary sex hormone and anabolic steroid in males. |
Alternative Name | ChEBI:17347 |
UniProt | |
Immunogen | This antibody was generated by panning against the VTT naïve human scFv phage display libraries VHVκ and VHVλ. |
Application Notes | The testosterone binding affinity of the 5F2 Fab fragment is 900 nM as determined by a competitive ELISA. Binding specificity of the 5F2 Fab was also studied by using 5α‐ dihydrotestosterone, dehydroepiandrosterone sulphate and androstenedione. Furthermore the Fab version of this antibody was co-crystallized with testosterone and the structure was identified using x-ray crystallography (Niemi et al, 2011; pmid:21360611). |
Antibody First Published | Niemi et al. The testosterone binding mechanism of an antibody derived from a naïve human scFv library. J Mol Recognit. Mar-Apr 2011;24(2):209-19. PMID:21360611 |
Note on publication | A testosterone binding scFv antibody was isolated from a naïve human library with a modest size of 100,000,000 clones. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
