Key features and details | |
Cat. No. | MABL-3040 |
Name | Anti-Tetanus toxoid mAbs |
Clone No. | AFD- 9F12 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, neutralize, ELISA |
Species Reactivity | Clostridium tetani |
Basic Information | |
Specificity | This antibody binds the intact or wild type tetanus toxin of Clostridium tetani. It was also reported to bind the tetanus toxin fragment B. Tetanus toxin is an extremely potent neurotoxin produced by Clostridium tetani. |
Alternative Name | TT; TTx; Tetanus toxin; tetX; Tentoxylysin |
UniProt | P04958 |
Immunogen | The original antibody was generated from the peripheral blood mononuclear cells form human donors after immunization with tetanus toxoids and fusing these lymphocytes with mouse myeloma line P3-X63-Ag 8.653. |
Application Notes | The screening of hybridoma supernatants to identify binding towards tetanus toxin was done using ELISA. The protective capacity of this antibody against tetanus toxoid was tested using the mouse tetanus toxin neutralization assay. The mice which received the tetanus toxin preincubated with 9F12 were completely protected (PMID: 7174795). The original antibody was used to study the role of gangliosides in the binding and internalization of bacterial toxins (PMID: 11093135). |
Antibody First Published | Gigliotti et al. Protective human hybridoma antibody to tetanus toxin. J Clin Invest. 1982 Dec;70(6):1306-9. PMID:7174795 |
Note on publication | Describes the generation and neutralization capacity of this antibody towards tetanus toxin. |
COA Information (For reference only, actual COA shall prevail) | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |