Key features and details | |
Cat. No. | MABL-3045 |
Name | Anti-Tetrabromobisphenol A mAbs |
Clone No. | AFD-T3-15 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | The antibody is highly selective for Tetrabromobisphenol A and it shows negligible cross reactivity (<0.1%) with other tested analogues (TBBPA-bAE, TBBPA-bOHEE, DBDPE, HBCD, BDE-47, BDE-99, BDE-100, BDE-153, BDE-154, BDE-183, 5-OH-BDE 47, 6-OH-BDE 47, BPA). Tetrabromobisphenol A is a ubiquitous brominated flame retardant. |
Alternative Name | TBBPA; 79-94-7; 3,3',5,5'-Tetrabromobisphenol A; Bromdian; 4,4'-(propane-2,2-diyl)bis(2,6-dibromophenol); 2,6-dibromo-4-[2-(3,5-dibromo-4-hydroxyphenyl)propan-2-yl]phenol; 2,2-bis(3,5-dibromo-4-hydroxyphenyl)propane; T15; brominated flame retardant |
UniProt | |
Immunogen | The original antibody was generated by immunizing an alpace with a TBBPA hapten coupled to thyroglobulin. A VHH phage display library was constructed with the blood lymphocytes and panning against heterogeneous coating antigens. |
Application Notes | The VHH fragment was used to develop an ELISA for monitoring environmental and human exposure to TBBPA. This assay had an IC50 for TBBPA of 0.40 ng/mL. Thermal stability studies showed the VHH−antigen binding signal retained about 80% and 20% of the unheated control signal after heating at 90 °C for 10 and 90 min, respectively. The recoveries of TBBPA from spiked soil and fetal bovine serum samples showed good correlation with liquid chromatography−tandem mass spectrometry method (Wang et al, 2014; PMID: 25068372). The VHH fragment was fused with alkaline phosphatase (AP) and a one-step immunoassay for the detection of TBBPA was developed, showing a half-maximum signal inhibition concentration (IC50) of 0.20 ng/mL. Compared to the parental VHH, the VHH-AP was able to bind to a wider variety of coating antigens and the assay sensitivity was slightly improved (Wang et al., 2015; PMID: 25849972). |
Antibody First Published | Wang et al. Heterologous Antigen Selection of Camelid Heavy Chain Single Domain Antibodies against Tetrabromobisphenol A Anal. Chem. 2014, 86, 16, 8296–8302 PMID:25068372 |
Note on publication | The paper describes the generation and characterization of the antibody. The antibody was used to develop an enzyme-linked immunosorbent assay for monitoring environmental and human exposure to TBBPA. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
