Key features and details | |
Cat. No. | MABL-3057 |
Name | Anti-Thiacloprid mAbs |
Clone No. | AFD- C4C4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | SPR, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody binds thiacloprid, which is a neonicotinoid insecticide. It is used in the production of a lot of agricultural crops and acts by affecting the insects nervous system. The antibody is also reported to have some cross reactivity with imidaclothiz and acetamiprid, slight cross reactivity with 2- chloro-5-[[2-(nitromethy-l-ene)-1-imidazolidinyl] methyl] pyridine (6-Cl-PMNI) and the thiacloprid amide (around 2%), and no obvious cross reactivity (<2%) with other structural analogues. |
Alternative Name | THD; neonicotinoid; pesticide; insecticide; CAS no: 111988-49-9; ChEBI: 391756; {(2Z)-3-[(6-Chloropyridin-3-yl)methyl]-1 3-thiazolidin-2-ylidene}cyanamide |
UniProt | |
Immunogen | The original antibody was generated by immunizing 6 weeks old BALB/c mice with a thiacloprid–bull serum albumin (BSA) conjugate. |
Application Notes | This antibody is recommended for detected of thiacloprid and other neonicotinoid pesticides. The binding characterization of this antibody to thiacloprid was done using ELISA. The recombinant antibody showed a binding profile similar to that of the original parental antibody. The reactivity of this antibody to other neonicotinoid pesticides was also determined using surface plasmon resonance. It was observed that thiacloprid and imidaclothiz displayed high binding responses, while imidacloprid, acetamiprid, and clothianidin showed medium binding responses. No significant binding was found toward dinotefuran, nitenpyram, and thiamethoxam, similar to the negative controls of two organophosphorus pesticides. (PMID: 32962080). |
Antibody First Published | Liu et al. Characterization of Variable Region Genes and Discovery of Key Recognition Sites in the Complementarity Determining Regions of the Anti-Thiacloprid Monoclonal Antibody. Int J Mol Sci. 2020 Sep; 21(18): 6857. PMID:32962080 |
Note on publication | Describees the use of next generation sequencing to characterize the variable regions of the antibody and a method of generation of sequence defined recombinant antibodies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
