Key features and details | |
Cat. No. | MABL-3080 |
Name | Anti-Tim-1 mAbs |
Clone No. | AFD- 3B3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | agonism, FC, IF, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for murine TIM-1, a member of the T cell immunoglobulin domain, mucin-like domain (TIM) gene family. TIM-1 has been found on activated CD4+ T cells, mast cells, and a subset of B cells. TIM-1 is involved in renal injury, tissue homeostasis, and T cell costimulation, and promotes the differentiation of Th2 cells and the production of IL-4. TIM-1 can bind several ligands, including Hepatitis A virus, phosphatidylserine, TIM4, IgA, as well as itself. |
Alternative Name | CD365; HAVcr-1; HAVCR1; Hepatitis A virus cellular receptor 1 homolog; Kidney injury molecule 1; KIM-1; T cell immunoglobulin and mucin domain-containing protein 1; T cell membrane protein 1; T-cell immunoglobulin and mucin domain containing 1; T-cell immunoglobulin mucin receptor 1; TIM-1; TIM1; TIMD-1 |
UniProt | Q5QNS5 |
Immunogen | This antibody was raised by immunising female Lewis strain rats (Harlan Sprague-Dawley) subcutaneously with mouse TIM-1–Ig in complete Freund's adjuvant (CFA), followed by multiple 'boosting' with mouse TIM-1–Ig in PBS. |
Application Notes | This antibody has been used in multiple FACS analyses, such as to determine if specific B cell subsets or if B cell-derived interleukin-10 contributes to tolerance (Lai et al, 2015), to test if blocking several checkpoint receptors boosts anti-tumor immunity in a low-dose, lympho-depleting whole body irradiation model (Jing et al, 2015), and to study the functions of kidney pericytes in vascular stability (Schrimpf et al, 2012). This antibody has also been used in immunohistochemistry to investigate the distinct role of matrix metalloproteinase-3 in TIM-1 shedding by kidney proximal tubular epithelial cells (Lim et al. 2012), and to demonstrate how Bβ(15-42) attenuates the effect of ischemia-reperfusion injury in renal transplantation (Sörensen et al, 2011). |
Antibody First Published | Umetsu Sarah et al. TIM-1 induces T cell activation and inhibits the development of peripheral tolerance. Nat Immunol. 2005 May;6(5):447-54. PMID:15793575 |
Note on publication | Describe the original generation of this antibody and its use in FACS analysis and in a TCR-transgenic DO11.10 mouse model to demonstrate that TIM-1 induces T cell activation and inhibits the development of peripheral tolerance. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
