Anti-Tissue factor mAbs

Cat. No.

MABL-3092

Name

Anti-Tissue factor mAbs

Clone No.

AFD-1157

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody binds mouse tissue factor (mTF) between amino acids 30 to 251. mTF hemostatic protein which is involved  in the extrinsic pathway of coagulation. TF is receptor for circulating   serine protease factor VIIa, activating the catalytic VIIa protease domain. The complex formed functions to activate the zymogen factor X into activated   factor Xa, which then catalyzes the formation of Prothrombin to Thrombin. The   pathways results in a fibrin clot which acts to stop bleeding. TF plays a role in normal hemostasis by initiating the cell-surface assembly and   propagation of the coagulation protease cascade.

Alternative Name

CD142; TF; mTF; F3; Coagulation factor III;   Thromboplastin; platelet tissue factor; factor III

UniProt

P20352

Immunogen

The original antibody was generated by   immunizing 6-week-old Wistar female rats intraperitoneally with recombinant   mouse tissue factor protein comprising amino acid sequence between 30 to 251   in Freund's complete adjuvant.

Application Notes

The initial binding characterization of the   hybridoma supernatant for mouse tissue factor was done using ELISA. The   binding was further confirmed with flow cytometry by measuring the reactivity   of the hybridoma culture supernatant to mTF-expressing cells. This antibody   was capable of being internalized by TF-forced-expression mouse B16 melanoma   cells expressing mTF (US9920133).

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.