Key features and details | |
Cat. No. | MABL-3105 |
Name | Anti-TLR3 mAbs |
Clone No. | AFD- 132-D4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | NTRL, ELISA |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for mouse TLR3. |
Alternative Name | Toll-like receptor 3; CD283 |
UniProt | Q99MB1 |
Immunogen | The original antibody was generated by immunizing llamas with a cocktail of murine and human Toll-like receptors (TLRs) that contained recombinant human TLR4/MD2, recombinant human TLR3, recombinant murine TLR3, and recombinant murine TLR2/Fc chimera. Peripheral blood mononuclear cells were isolated from the animals' blood for the construction of a phage display library, and the library was panned against the TLRs used for immunization. |
Application Notes | The binding specificity of the original version of this antibody (VHH) was tested using ELISA. In a cell-based neutralizing assay with mTLR3-overexpressing HEK cells, an antagonistic effect was observed, as measured by NFκB expression after cell lysis compared to the control group (WO2009138494A2). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
