Key features and details | |
Cat. No. | MABL-3116 |
Name | Anti-TMEM30A mAbs |
Clone No. | AFD-FC5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vitro, in vivo, Transcytosis, ELISA |
Species Reactivity | Dog, Rat, Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for human cerebral endothelial cells (HCEC), targeting TMEM30A as its putative receptor. It cross-reacts with mouse, rat, and dog endothelial cells. |
Alternative Name | TMEM-30A; Cdc50a; putative TMEM30A; HCEC; human cerebromicrovascular endothelial cells |
UniProt | |
Immunogen | The original antibody was selected from a llama-derived nanobody phage display library by phenotypic screening against human brain endothelial cells. |
Application Notes | The original antibody (llama VHH) bound specifically to HCEC, as demonstrated by an ELISA, and showed selective binding to brain endothelial cells (HCEC and HPEC). It localized to vesicular and membrane fractions, was internalized via an energy-dependent pathway without observed toxicity, and exhibited uptake into brain endothelial cells. A fusion protein version of this antibody (FC5-c-Myc-His5) demonstrated effective transmigration across an in vitro blood-brain barrier (BBB) model using HCEC monolayers, comparable to empty membranes, without altering BBB integrity. In vivo, the antibody accumulated in brain tissue after intravenous injection (Muruganandam et al., 2002; PMID: 11772942). This antibody's ability to facilitate transcytosis across the BBB via receptor-mediated pathways was demonstrated through significantly enhanced brain and cerebrospinal fluid (CSF) exposure in rat and dog in vivo models. |
Antibody First Published | Muruganandam et al., Selection of phage-displayed llama single-domain antibodies that transmigrate across human blood-brain barrier endothelium. FASEB J. 2002 Feb;16(2):240-2. doi: 10.1096/fj.01-0343fje. PMID:11772942 |
Note on publication | The original publication describes the selection and characterization of llama single-domain antibodies (sdAbs) capable of selectively binding to human cerebromicrovascular endothelial cells and transmigrating across the blood-brain barrier. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
