Key features and details | |
Cat. No. | MABL-3118 |
Name | Anti-TNFalpha mAbs |
Clone No. | AFD- 2TY |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This scFv is specific for human TNF-α. |
Alternative Name | TNFSF2; tumor necrosis factor alpha; TNF; C1K3N5; TNFA; Cachectin; TNF-alpha; TNF-a; Tumor necrosis factor ligand superfamily member 2; TNF-α |
UniProt | P01375 |
Immunogen | Affinity selection procedures were used to isolate this scFv from a naïve Tomlinson I phage display library that specifically binds to TNF-α.§ The panning procedure was carried out by incubating immunotubes coated with recombinant TNF-α protein with a phage suspension. Phage particles with affinity for recombinant TNF-α were amplified in TG-1 bacteria, and three rounds of panning were performed to select high affinity and specificity scFvs. |
Application Notes | The binding affinity of this scFv for human TNF-α has been confirmed in ELISA analysis (Safarpour et al, 2018). This scFv could have future therapeutic applications as a TNF inhibitor. TNF inhibition is a strategy currently showing promise in the treatment of a range of inflammatory diseases, including rheumatoid arthritis and Chron's disease. |
Antibody First Published | Safarpour et al. Isolation and Characterization of Novel Phage Displayed scFv Fragment for Human Tumor Necrosis Factor Alpha and Molecular Docking Analysis of Their Interactions Iran J Pharm Res. 2018 Spring; 17(2): 743–752. PMID:29881431 |
Note on publication | Describes the original generation of this scFv by phage display. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
