Key features and details | |
Cat. No. | MABL-3125 |
Name | Anti-toxin A mAbs |
Clone No. | AFD- A26.8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | BLI, neutralizing, SPR, ELISA |
Species Reactivity | Clostridium difficile |
Basic Information | |
Specificity | The antibody is specific for toxin A of Clostridium difficile. It recognises a conformational epitope on native C. difficile toxin. |
Alternative Name | TcdA; EC:3.4.22 |
UniProt | P16154 |
Immunogen | The original antibody was generated by immunizing a llama with recombinant RBD fragments TcdA-RBD-f1 and TcdB-RBD-f1, followed by phage display library construction and panning against TcdA- RBD-f1. |
Application Notes | The specificity of the original format of the antibody to TcdA was confirmed by ELISA analysis. The binding affinity of the original format of the antibody was measured by surface plasmon resonance (Kd= 12 nM). Human lung fibroblast (HLF) cytotoxicity assay was used to determine whether the original format of the antibody could neutralize TcdA-induced HLF cell rounding. The antibody showed neutralizing activity to TcdA (US9771416B2). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
